Plasmid Preparation:Article Title: IQGAP1 is associated with nuclear envelope reformation and completion of abscission
Article Snippet: .. Plasmid constructs GST-IQGAP1 (human sequence, pGEX-2T vector; Addgene plasmid 30107) and GFP-IQGAP1 (human sequence, EGFP-C2 vector) plasmids were described previously. .. 83 Phospho-deficient (S864A, S330A and T1434A) and phospho-mimetic (S864E, S330E and T1434E) mutant forms of GFP-IQGAP1, phospho-deficient forms of GST-IQGAP1, as well as siRNA resistant forms of all GFP-IQGAP1 constructs were generated using the QuickChange site-directed mutagenesis kit (Stratagene).
Article Title: IQGAP1 is associated with nuclear envelope reformation and completion of abscission.
Article Snippet: .. Plasmid constructs GST-IQGAP1 (human sequence, pGEX-2T vector; Addgene plasmid 30107) and GFP-IQGAP1 (human sequence, EGFPC2 vector) plasmids were described previously.83 Phospho-deficient (S864A, S330A and T1434A) and phospho-mimetic (S864E, S330E and T1434E) mutant forms of GFP-IQGAP1, phospho-deficient forms of GST-IQGAP1, as well as siRNA resistant forms of all GFP-IQGAP1 constructs were generated using the QuickChange site-directed mutagenesis kit (Stratagene). ..
Construct:Article Title: IQGAP1 is associated with nuclear envelope reformation and completion of abscission
Article Snippet: .. Plasmid constructs GST-IQGAP1 (human sequence, pGEX-2T vector; Addgene plasmid 30107) and GFP-IQGAP1 (human sequence, EGFP-C2 vector) plasmids were described previously. .. 83 Phospho-deficient (S864A, S330A and T1434A) and phospho-mimetic (S864E, S330E and T1434E) mutant forms of GFP-IQGAP1, phospho-deficient forms of GST-IQGAP1, as well as siRNA resistant forms of all GFP-IQGAP1 constructs were generated using the QuickChange site-directed mutagenesis kit (Stratagene).
Article Title: IQGAP1 is associated with nuclear envelope reformation and completion of abscission.
Article Snippet: .. Plasmid constructs GST-IQGAP1 (human sequence, pGEX-2T vector; Addgene plasmid 30107) and GFP-IQGAP1 (human sequence, EGFPC2 vector) plasmids were described previously.83 Phospho-deficient (S864A, S330A and T1434A) and phospho-mimetic (S864E, S330E and T1434E) mutant forms of GFP-IQGAP1, phospho-deficient forms of GST-IQGAP1, as well as siRNA resistant forms of all GFP-IQGAP1 constructs were generated using the QuickChange site-directed mutagenesis kit (Stratagene). ..
Sequencing:Article Title: IQGAP1 is associated with nuclear envelope reformation and completion of abscission
Article Snippet: .. Plasmid constructs GST-IQGAP1 (human sequence, pGEX-2T vector; Addgene plasmid 30107) and GFP-IQGAP1 (human sequence, EGFP-C2 vector) plasmids were described previously. .. 83 Phospho-deficient (S864A, S330A and T1434A) and phospho-mimetic (S864E, S330E and T1434E) mutant forms of GFP-IQGAP1, phospho-deficient forms of GST-IQGAP1, as well as siRNA resistant forms of all GFP-IQGAP1 constructs were generated using the QuickChange site-directed mutagenesis kit (Stratagene).
Article Title: IQGAP1 is associated with nuclear envelope reformation and completion of abscission.
Article Snippet: .. Plasmid constructs GST-IQGAP1 (human sequence, pGEX-2T vector; Addgene plasmid 30107) and GFP-IQGAP1 (human sequence, EGFPC2 vector) plasmids were described previously.83 Phospho-deficient (S864A, S330A and T1434A) and phospho-mimetic (S864E, S330E and T1434E) mutant forms of GFP-IQGAP1, phospho-deficient forms of GST-IQGAP1, as well as siRNA resistant forms of all GFP-IQGAP1 constructs were generated using the QuickChange site-directed mutagenesis kit (Stratagene). ..
Mutagenesis:Article Title: IQGAP1 is associated with nuclear envelope reformation and completion of abscission.
Article Snippet: .. Plasmid constructs GST-IQGAP1 (human sequence, pGEX-2T vector; Addgene plasmid 30107) and GFP-IQGAP1 (human sequence, EGFPC2 vector) plasmids were described previously.83 Phospho-deficient (S864A, S330A and T1434A) and phospho-mimetic (S864E, S330E and T1434E) mutant forms of GFP-IQGAP1, phospho-deficient forms of GST-IQGAP1, as well as siRNA resistant forms of all GFP-IQGAP1 constructs were generated using the QuickChange site-directed mutagenesis kit (Stratagene). ..
Generated:Article Title: IQGAP1 is associated with nuclear envelope reformation and completion of abscission.
Article Snippet: .. Plasmid constructs GST-IQGAP1 (human sequence, pGEX-2T vector; Addgene plasmid 30107) and GFP-IQGAP1 (human sequence, EGFPC2 vector) plasmids were described previously.83 Phospho-deficient (S864A, S330A and T1434A) and phospho-mimetic (S864E, S330E and T1434E) mutant forms of GFP-IQGAP1, phospho-deficient forms of GST-IQGAP1, as well as siRNA resistant forms of all GFP-IQGAP1 constructs were generated using the QuickChange site-directed mutagenesis kit (Stratagene). ..
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